Furthermore, cellular Daxx levels decreased markedly after the heat shock induction of HSP25 expression in these mouse cancer cells (Fig. 3b), and transcriptional repression of Daxx was triggered by the transcriptional induction of HSP25 in these cells, suggesting that Daxx expression is inversely proportional to HSP25 gene expression (Fig. 3c). This evidence concerns the gene HSPB1 and cancer.