Consistent with the in vivo reduction of musclin in cachexia, myotubes expressing caFoxO3 for 24, 48 and 72 h display progressively reduced expression of musclin, unlike the other myokines that were mostly unchanged, with the exception of NppB mainly induced during atrophy (Figure 5c) and FNDC5 reduced at 24 and 48 h (Figure S2d). The gene discussed is NPPB; the disease is Cachexia.