The Wnt, p53, and receptor tyrosine kinase (RTK)/Ras pathways displayed similar alterations in CRC cells as in patient samples, whereas phosphatidylinositol-3-kinase (PI3K) and TGFβ pathways were mutated in CRC-derived cell lines with significantly lower frequencies than in tumor specimens. This evidence concerns the gene TGFB1 and neoplasm.