In the present study, individuals with CNVs including SLIT3 were reported with malformation of the heart and great vessels (n=1), ventricular septal defect (n=1), atrial septal defect (n=3), and TOF (n=1) whereas individuals with SLIT2 CNVs were reported with malformation of the heart and great vessels (n=1), ventricular septal defect (n=2), and double outlet right ventricle (n=1). Here, SLIT3 is linked to Abnormal cardiac septum morphology.