There were a number of genes that already had an assigned phenotype (Online Mendelian Inheritance in Man)36; among these, 59 had been previously associated with CHD pathogenesis such as ZIC3, NKX2-6, GATA4, JAG1, GJA1, and TBX5. All genes with Online Mendelian Inheritance in Man assigned phenotypes were excluded from further analysis. This evidence concerns the gene GJA1 and coronary artery disorder.