Priming with B23-C20 peptide provided the most overt disease exacerbation, with very rapid development of diabetes (100% incidence by day 84 post prime; mean ± SD onset 49 ± 16.2 days post prime), characterised by severe leucocyte infiltration in the pancreatic islets (ESM Fig. 1b). The gene discussed is NPM1; the disease is diabetes mellitus.