However, in the presence of oxidative stress induced by 2DG (Simons et al., 2009) and NDV (Al-Shamery et al., 2012; Cheng et al., 2016) treatment and glutathione (GSH) depletion due to the accumulation of reactive oxygen species (ROS), the activity of glyoxalase 1 (Glo1) declined, leading to the accumulation of methylglyoxal, which has a direct cytotoxic effect on cancer cells (Thornalley and Rabbani, 2011). Here, GLO1 is linked to cancer.