Several factors have been identified playing a role behind response to checkpoint blockade: tumor mutation burden (88), PDL1 expression (89, 90), T cell inflamed microenvironment (91), T cell repertoire richness and clonality (92), HLA-I diversity (93), intestinal microbiota (94, 95), and specific mutations have all been identified as potential markers with prognostic or predictive value in checkpoint blockade therapy (87). The gene discussed is CD274; the disease is neoplasm.