The effects of short-term insulin exposure observed in our study does not contradict the results of intranasal insulin therapy, which has already been shown to be effective in the treatment of Alzheimer’s disease (Craft et al., 2012) and experimental traumatic brain injury (Brabazon et al., 2017), for which glutamate excitotoxicty is an accompanying condition or symptom. This evidence concerns the gene INS and Alzheimer disease.