At days 38 and 43, we also found an overrepresentation of sgRNAs to LSCP-HK TFs (IRF6, RELA, STAT1, ERF, TGIF1, VDR, RUNX1, ESSRA, and IRF2) implicating these CRC-TFs in the loss of stem cell phenotype with time in vitro (Fig. 4b). This evidence concerns the gene ERF and colorectal carcinoma.