Existence of three subsets of intratumoral Mo/MΦs identified in the present study is in line with the finding that tissue resident macrophages differentiate in various physiological states29, which is supported by the findings that monocytes transform their surface characteristics after infiltration into tissues30 and that SIRPα expression was downregulated when co-cultured with tumor cells31. This evidence concerns the gene SIRPA and neoplasm.