Since CBFβ showed higher expression in metastasis cell lines MNNH/HOS and 143B as compared with non-metastatic cell lines U-2OS and KHOS, we examined whether CBFβ knockdown by siRNA transfection could affect the migratory and invasive activities of osteosarcoma cells by wound healing assay and Matrigel invasion assay. Here, CBFB is linked to osteosarcoma.