We therefore determined if C. rodentium can activate ALPK1 by analysing formation of TIFA oligomers, also called “TIFAsomes,” following infection of TIFA‐GFP expressing reporter HeLa cells with wild type (WT), C. rodentium ΔescN (T3SS deficient), ΔhldE, and ΔrfaC; treatment with ADP‐hep (10−7 M) was used as a positive control. Here, ALPK1 is linked to infection.