Therefore, we performed an antigen-specific CD8+ T cell proliferation assay using Influenza A hemagglutinin (HA)-specific TCRtg CD8+ T cells to mimic T cell priming during Influenza infection and ovalbumin (OVA)-specific TCRtg CD8+ T cells (OT-I) as one of the best characterized model antigen. This evidence concerns the gene CD8A and influenza.