Immunoblot analysis of infected-cell lysates showed that the overall amounts of endogenous TLR3 and TLR4 proteins, but not another membrane protein, Calnexin, decreased considerably after infection with wild-type HCMV, HCMVΔUS7-16-Rev.US7, or HCMVΔUS7-16-Rev.US8, whereas they remained unchanged in cells infected with HCMVΔUS7-16 (Fig. 6e). This evidence concerns the gene TLR4 and infection.