Although depletion of mtDNA did not change the expression levels of DDX41, RIG-I, MAVS, and STING (Supplementary Fig. 13c), ρ0 HEK293FT cells significantly reduced the IFN-β gene expression after infection with ΔNS1 influenza virus (Supplementary Fig. 13d). This evidence concerns the gene IFNB1 and infection.