Finally, to further address the possible role of TfR in NLC uptake, we compared NLC uptake by two epithelial human cell lines, one with high expression of TfR (MCF-7, a human breast cancer cell line) and the other with poor expression of TfR (MCF-10A, a non-tumourigenic epithelial cell line of mammary gland/breast origin, which expresses only 10% of TfR compared with MCF-7 cells). This evidence concerns the gene TFRC and breast cancer.