Consistent with the RNA-seq analysis, ZC3H18 depletion profoundly decreased BRCA1 mRNA (Fig. 2b; Supplementary Fig. 4a) and protein levels (Fig. 2a) in multiple ovarian cancer cell lines and in xenografted OVCAR-8 cells (Supplementary Fig. 2c). This evidence concerns the gene BRCA1 and ovarian cancer.