NF1 and glioblastoma: Among the genes with a mutation detected, NF1 has the highest number of pathogenic variants (six total), and the alterations (three nonsense mutations, one splicing donor change, and two common missense mutations) have low VAFs (3.5%–15.3%), consistent with the growth advantage from NF1 defects in the later stage of GBM development (Philpott et al. 2017).