Moreover, we also observed consistent phenomena in HSV-1-infected A549 cells with IFI16-/-, IFI16-/—STING knockdown or IFI16-/—cGAS knockdown (S5B Fig), indicating that IFI204 might facilitate cGAS-STING DNA sensing pathway that leads to IRF3 activation during the infection of HSV-1. Here, IFI16 is linked to infection.