We examined DNMT1, DNMT3a and DNMT3b (DNA methyltransferase 1, 3 alpha and 3 beta), and we found that DNMT3a was significantly upregulated after 24 hours in IPF fibroblast and after 5-day treatment in both cell lines (Fig 3B, p<0.05). The gene discussed is DNMT1; the disease is idiopathic pulmonary fibrosis.