PPARD and malignant colon neoplasm: In this latter study, it was found that although IHC analyses detected lower PPARβ/δ expression in colon tumors from mice treated with an NSAID,41 quantitative western blot analyses revealed that there was in fact no change in relative expression of PPARβ/δ between control and NSAID-treated colon tumors (or non-transformed tissue).31 Additional studies based on dose-dependent analyses of NSAID-induced inhibition of human colon cancer cell lines also revealed no decrease in expression of PPARβ/δ by NSAIDs at concentrations that inhibited cell proliferation of these colon cancer cell lines.