To examine whether ACM upregulates ANGPTL4 expression in other TNBC cells, TNBC MDA-MB-231, MDA-MB-468, HCC1937 cells, breast cancer estrogen receptor-positive cells (MCF-7), and immortalized breast epithelial cells (MCF-10A) were sequentially passaged in ACM for five passages. The gene discussed is ANGPTL4; the disease is breast cancer.