Following the demonstration of the low TGF-β2 expression level in tumor-conditioned medium (TCM) from the cultures of TNBC MDA-MB-231, MDA-MB-468, and HCC1937 cells in serum-free breast cancer cell line media (Fig. 5a left panel), the media were used in culture with astrocytes, respectively. This evidence concerns the gene TGFB2 and neoplasm.