Here, we constructed the model for induction of lung cancer stemness, including elevated sphere-forming and colony forming capabilities, increased expression of CSC-associated markers (CD133 and CD44) and multipotential transcription factors (Bmi1 and Oct4) by chronic inflammation using co-treatment with TNF-α and TGF-β to NSCLC H460 cells. This evidence concerns the gene POU5F1 and non-small cell lung carcinoma.