In the diagnostic analysis of the HLA family, HLA-A, HLA-C, HLA-DMB, HLA-DOA, HLA-DOB, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB4, HLA-DRB6 and HLA-E showed significance for HCC diagnosis (all P ≤ 0.05, Figure 3A, C, E-K, M-Q). The gene discussed is HLA-DOB; the disease is hepatocellular carcinoma.