For this purpose, AEBP1 was silenced in U138MG glioma cells using 100 nM AEBP1 siRNA pool, which was replenished every 36 hours over a period of 9 days and assessed for cellular viability MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)assay. The gene discussed is AEBP1; the disease is glioma.