GYPA and infection: Next, we performed a pooled lv-based CRISPR–Cas9 screen targeting 19,050 genes and 1864 miRNAs27 in HEL cells using two rounds of antibody-based CD235a+ cell depletion to derive a population of CD235a−/low cells (day 12 post-infection) (Fig. 1a, and Supplementary Fig. 1d).