In this study, we further demonstrate that BEFV induces autophagy via upregulation of the PI3K/Akt/NF-κB and the Src/JNK/AP1 pathways in the early to middle stages of infection and causes suppression of the PI3K/Akt/mTORC1 pathway at the late stage of infection, all of which benefit virus replication. This evidence concerns the gene MAPK8 and infection.