TILs were expanded with the REP (rapid expansion protocol) from tumoral or inflamed/normal prostate (n = 8), colon cancer metastasis (n = 4) and metastatic melanoma (n = 6) (Additional file 2: Table S2); cell cultures with heterogeneous CD4+/CD8+ T cell ratios were obtained according to the tumor evaluated (Additional file 1: Figure S7a). Here, CD8A is linked to malignant colon neoplasm.