Additionally, CD4 + CD25 + Treg cells secrete TGF-β and IL-10 to suppress effector T cells such as CD8 + cytotoxic T lymphocytes infiltrating the tumor into HCC secreting anti-tumor effector molecules such as IFN -γ, IL-2 and TNFα [55,56]. This evidence concerns the gene TNF and hepatocellular carcinoma.