Animal experiments showed that the tumour weight of the vector-TTN-AS1 + sh-NC group (Saos-2: 2.66 ± 0.69; U-2OS: 2.24 ± 0.43) was significantly higher than that of the vector-NC group; the cell viability of the vector-TTN-AS1 + sh-MBTD1 group (Saos-2: 0.99 ± 0.18; U-2OS: 0.76 ± 0.11) was lower than that of the Vector-TTN-AS1 + sh-NC group (Figure 6E–6F). This evidence concerns the gene MBTD1 and neoplasm.