Another study investigated DNA methylation in CD4+ T cells from JIA patients; 145 DMPs were identified between cases and controls (false discovery rate adjusted P < 0.1), but just 11 DMPs remained associated when four MTX-treated individuals were removed, suggesting a possible role of MTX on DNA methylation in CD4+ T cells in JIA cases [9]. This evidence concerns the gene CD4 and juvenile idiopathic arthritis.