In this study, we confirm that GLUT1 mRNA expression in bronchial brushing samples of NSCLC is significantly higher than that in benign group and GLUT1 promotes the malignant phenotype of NSCLC through integrin β1/Src/FAK signaling, which provides a new target for the research and treatment of lung cancer. This evidence concerns the gene SRC and non-small cell lung carcinoma.