To exclude a potential confounding effect of the patients’ liver function or alpha-fetoprotein (AFP) serum levels in the subgroup of HCC patients, we performed Cox-regression analysis which revealed no impact of bilirubin (HR: 0.634 (0.237–1.679), p = 0.365) or AFP (HR: 1.000 (0.999–1.001), p = 0.412) serum levels on patients’ overall survival in our cohort. This evidence concerns the gene AFP and hepatocellular carcinoma.