Consistent with the results of western blotting, the result of ELISA confirmed that the protein level of RANKL was not increased in RiCKO cellular supernatant as much as in Rictorf/f supernatant after treatment with TCM (Fig. 3G).These results demonstrate that Rictor is crucial for TCM-induced RANKL expression in BMSCs, suggesting that reduction of RANKL expression by BMSCs in RiCKO mice is a major mechanism for the decreased osteoclastogenesis induced by cancer cells. The gene discussed is RICTOR; the disease is cancer.