Synergistic effects of anti-oxidant agents and chemotherapeutic drugs (such as combination of gemcitabine, 5-FU, and cisplatin) resort on induction of apoptosis, inhibition of cell proliferation and metastasis invasion.16-18 Chemo-herbal combination therapy does attenuate the hazards to inducing drug resistance and prevalence of chemotherapy side effects.19 The present study was designed to investigate the synergistic effects of rutin and 5-FU chemo-herbal combination on apoptosis, colony formation, p53 gene expression, and Bcl-2 signaling protein in PC3 prostatic cancer cells. Here, TP53 is linked to prostate carcinoma.