In addition, qRT-PCR analyses revealed a comparable Grm1 mRNA expression level of cultivated Tg(Grm1) cell lines to murine cerebellum (positive control, set as 1), whereas the murine B16 melanoma and melanocytic Melan-A cell line (negative control) displayed extremely low Grm1 expression (Fig. 1f). The gene discussed is GRM1; the disease is melanoma.