Further immunoblot assays showed that T24 cells expressed the highest TAGLN protein levels among the four carcinoma cell lines (Figure 1B) which were similar to the results of RT-qPCR assays presented in the Figure 1A. The immunoblot assays also revealed that HBdSMC cells expressed higher protein levels of alpha-smooth muscle actin (α-SMA), and HBdEC cells exhibited higher protein levels of uroplakin-2 (UPK-2), a marker of bladder transitional cells (Figure 1C). Here, ACTA1 is linked to carcinoma.