The RT-qPCR analysis of paired human bladder tissues showed that means of ΔΔCt between normal and cancer tissues were 3.77 ± 0.67 using β-actin as internal control (Figure 1E) and 4.33 ± 0.72 using 18S as internal control (Figure 1F), respectively, suggesting significantly higher expressions of TAGLN mRNA levels in normal bladder tissues than that in bladder cancer tissues. The gene discussed is TAGLN; the disease is cancer.