ACTB and cancer: The RT-qPCR analysis of paired human bladder tissues showed that means of ΔΔCt between normal and cancer tissues were 3.77 ± 0.67 using β-actin as internal control (Figure 1E) and 4.33 ± 0.72 using 18S as internal control (Figure 1F), respectively, suggesting significantly higher expressions of TAGLN mRNA levels in normal bladder tissues than that in bladder cancer tissues.