To understand the regulatory role of CyclinD1 in Dicer expression, we first tested the relative levels of CyclinD1 and CyclinD1 expression in ICC HuccT-1, HCCC9810 and RBE as well as non-tumor cholangiocyte HIBEpic cells by qRT-PCR and Western blot assays. The gene discussed is CCND1; the disease is intrahepatic cholangiocarcinoma.