Based on previous studies to optimize dose and duration of a HF diet in mouse [48,49,50], 6-week-old KRIT1+/− mice (n = 6) and wild-type littermates (n = 6) were fed a HF diet for 22 weeks and analyzed for fatty streaks deposition and VCAM-1 mRNA expression in atherosclerosis-susceptible (aortic arch and aortic root) and -protected (descending thoracic aorta) regions of the aorta (Figure 2A) [51,52,53]. The gene discussed is KRIT1; the disease is atherosclerosis.