In this way, to determine whether the pure compounds induce apoptotic cell death by regulating antiapoptotic (Bcl-2) and proapoptotic (Bax) proteins in the hepatocellular carcinoma Hep3B/PTX cells, expression of Bax and Bcl-2 was analyzed by RT-PCR. This evidence concerns the gene BCL2 and hepatocellular carcinoma.