Moreover, the loss of MMP was accompanied by a down-regulation in the Bcl-2/Bax ratio and the promotion of cytosolic release of cytochrome c. Therefore, based on those observations, we speculated that the pro-apoptotic effect of isorhamnetin in bladder cancer cells could occur by simultaneously activating extrinsic and intrinsic pathways. The gene discussed is CYCS; the disease is urinary bladder cancer.