Here, we describe MERG’s methods for integrating complex longitudinal data from qRT-PCR, IgM, IgG3, and PRNT50 assays in order to establish case definitions for ZIKV infections in pregnancy, discuss the advantages and limitations of this approach, and provide specific recommendations for future prospective cohort studies of ZIKV infections in pregnancy. Here, CD40LG is linked to Zika virus infectious disease.