To identify the molecular pathway mediated by VSTM2A, we screened the important cancer signaling pathways by luciferase reporter assays, including Wnt/β-catenin, NF-кB, MAPK, TGF-β, JNK, STAT3, PI3K/AKT, BMP, and p53. The gene discussed is TGFB1; the disease is cancer.