Finally, we tested the relevance of Nrg1 intracellular signaling upon stroke in vivo by performing the stereotactic injection of AAVs expressing either Nrg1-ICD or GFP as a control in mouse brain cortices and then using a focal hemorrhagic stroke to provoke an infarct adjacent to the cortical region expressing Nrg1-ICD or GFP. Here, NRG1 is linked to hemorrhagic stroke.