Immunohistochemistry (IHC) staining showed increased autophagy-related proteins expression in the tumor isolated from the mice with injection of circCDR1as cells including LC3B, p62 of cytoplasm, LAMP2, and ATG5, and the expression of HIF-1α were increased in experiment groups compared to the control groups (Fig. 6d, e). This evidence concerns the gene MAP1LC3B and neoplasm.