To further verify the role of the PERK-eIF2α-ATF4-CHOP pathway in the 125I and LBP coinduced apoptosis and anti-proliferation of HCC cells, cell cycle (B), Bax/Bcl-2 ratio (C), and Annexin V–APC/PI assays (D) were performed. This evidence concerns the gene ATF4 and hepatocellular carcinoma.