While Dsg3 staining with rabbit antibody (H145) that binds to its C-terminus exhibited broad variations, another mouse antibody (5H10) that binds the N-terminus at the extracellular domain of Dsg3 showed marked depletion of Dsg3 from the surface in cells treated with PV sera (Fig. 6a, b). This evidence concerns the gene DSG3 and acquired polycythemia vera.