MIEN1 and Familial prostate cancer: The immunohistochemical assay (IHC) results of a human prostate tissue array are demonstrated in Figure 1C. The immunostaining for MIEN1 in normal (n = 9), grade II (n = 8), and grade III (n = 32) prostate cancer tissues was determined by immunohistochemistry assays in a human prostate tissue array.