Significantly decreased CPT1A in ADH− versus ADH+ deer mice fed 3.5% EtOH and decreased ratios for p-AMPK/AMPK and p-ACC1/ACC1 could be key determinants for increased EtOH-induced hepatic steatosis in ADH− deer mice fed 3.5% EtOH. Here, ACACA is linked to Hepatic steatosis.