The confocal microscopy images showed that not only an increase in the number of GFP-LC3-positive vesicles was detected, which represents the formation of autophagosomes, but also the events of atto647N-DENV co-localization with autophagosomes elevated in both mock (siRNA-free) and si-control groups, as compared to those at the initial time point of infection (Figure 3D). Here, MAP1LC3A is linked to infection.