MAP1LC3A and hepatocellular carcinoma: To reveal the roles of TIM-1 on DENV-induced autophagy, immunofluorescence staining first confirmed the expression of TIM-1 in Huh7.5 cells, which is a DENV highly permissive cell lines derived from cellular hepatocellular carcinoma [10,49], and GFP-LC3 stably expressing Huh7.5 cells (GFP-LC3-Huh7.5).